Figure 3.

Activated hepatic CCR6⁺ ILC3s proliferate in response to partial hepatectomy

(A) Cellular changes compared with sham-operated mice of different ILC subsets analyzed by flow cytometry in livers of SPF wild-type mice 24 h after PH (aritmetic mean ± SD). ILC1s, Lin⁻CD127⁺RORγt⁻Tbet⁺; ILC2s, Lin⁻CD127⁺RORγt⁻GATA⁺; ILC3s, Lin⁻CD127⁺RORγt⁺; NK cells, Lin⁻CD127⁻NKp46⁺; Th17 cells, CD19⁻CD3⁺RORγt⁺.

(B) Flow cytometric quantification and statistical analysis of liver RORγt⁺ILC3s 24 h after PH or sham surgery.

(C–G) Rorc(γt)^Cre−TgRosa26R^EYFP/+ (RORγtFM) mice underwent PH and were analyzed by flow cytometry. (C and D) ILC3s and their subsets at 24 h post PH. (E) Proliferation of CCR6⁺ ILC3s was assessed by Ki-67 24 h after PH. (F) Sort purification of RORγt⁺ ILC3s and measurement of IL-22 by qPCR (aritmetic mean ± SD). (G) Flow cytometry analysis of IL-22 production by CCR6⁺ ILC3s. Geometric means for log scales and arithmetic means for linear scales are shown. Normalized values were analyzed by Student’s t test to compare two experimental groups or by ANOVA to compare more than two groups in parallel. Data in (A)–(G) are representative of n = 3–4 mice per group in two or more independent experiments. The p values are indicated as follows: ^∗p ≤ 0.05. See also Figures S2 and S3 and Table S1.