Figure 5.

ILC3s regulate the hepatocyte-derived antimicrobial peptide production

(A) SPF Il22^+/+ and Il22^−/− mice underwent PH. Antibiotic-resistant E. coli JM83 was i.g. delivered 12h before PH. E. coli titers (CFU) were assessed in the liver and spleen 24 h after PH.

(B–G) Whole-tissue RNA was isolated from the liver and the expression of antimicrobial peptide-encoding genes was analyzed by RT-qPCR at the indicated time points. (B and C) SPF and germ-free wild-type mice. (D and E) Anti-Thy1.2 antibody or anti-IgG in (D) SPF wild-type and (E) SPF Rag 1^−/− mice. (F) SPF Rorc(γt)^+/+ and Rorc(γt)^−/− mice. (G) Colonized Asc^+/+ mice and Asc^−/− mice.

(H) t-SNE dimensionality reduction visualization of single-cell RNA sequencing of purified liver cells showing the main cell types.

(I and J) Dropout corrected gene expression of Reg3b (aritmetic mean ± SD) at different time points after hepatectomy. Geometric means for log scales and arithmetic means for linear scales are shown. Normalized values were analyzed by Student’s t test to compare two experimental groups or by ANOVA to compare more than two groups in parallel. Data in (B)–(G) are representative of n = 3–12 mice per group in two or more independent experiments, and data in (A) are pooled based on n = 6–7 mice from two independent experiments. The p values are indicated as follows: ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001, and ^∗∗∗∗p ≤ 0.0001. See also Figures S5 and S6.