| Coating Ab | Clone | Brand | Concentration |
|---|---|---|---|
| Purified Rat Anti-Human and Viral IL-10 | JES3-9D7 | BD Biosciences | 1 μg/mL |
| Purified Mouse Anti-Human IFNg | NIB42 | BD Biosciences | 1 μg/mL |
| Detection Ab | Clone | Brand | Concentration |
| Biotin Anti-Human and Viral IL-10 | JES3-12G8 | BD Biosciences | 1 μg/mL |
| Biotin Anti-Human IFNg | 4S.B3 | BD Biosciences | 1 μg/mL |
| Standard Curve | Brand | Concentration (max:min) | |
| Recombinant Human IL-10 protein | – | CellGenix | 2000 : 15,625 pg/mL |
| Recombinant Human IFNg | – | R&D Systems | 2000 : 15,625 pg/mL |
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