Table 1. Primers used for PCR amplification of β-lactamase and mcr-1 genes.
Gene | Primer sequence (5′–>3′) | T∘ annealing (° C) | Amplicon length | Reference |
---|---|---|---|---|
bla TEM | F: ATGAGTATTCAACATTTTCG R: TTACCAATGCTTAATCAGTGAG |
55 °C | 861 bp | Alcántar-Curiel et al. (2019b) |
bla SHV | F: ATGCGTTATATTCGCCTGTGTATT R: TTAGCGTTGCCAGTGCTCGATC |
60 °C | 861 bp | Alcántar-Curiel et al. (2019b) |
bla CTX-M | F: CGCTTTGCGATGTGCAG R: ACCGCGATATCGTTGGT |
52 °C | 550 bp | Alcántar-Curiel et al. (2019b) |
bla KPC | F: TCACTGTATCGCCGTCTAGTTCTG R: TTACTGCCCGTTGACGCCCAATC |
58 °C | 875 bp | Alcántar-Curiel et al. (2019b) |
bla V IM | F: GAGTGGTGAGTATCCGACAGTCAACGAAAT R: AGAGTCCTTCTAGAGAATGCGTGGGAATCT |
58 °C | 389 bp | Alcántar-Curiel et al. (2019b) |
bla IMP | F: GCATTGCTACCGCAGCAGAGTCTTTG R: GCTCTAATGTAAGTTTCAAGAGTGATGC |
58 °C | 647 bp | Alcántar-Curiel et al. (2019a) |
bla NDM | F: GTCTGGCAGCACACTTCCTATCTC R: GTAGTGCTCAGTGTCGGCATCACC |
58 °C | 516 pb | Alcántar-Curiel et al. (2019b) |
bla OXA-23 | F: GATCGGATTGGAGAACCAGA R: ATTTCTGACCGCATTTCCAT |
52 °C | 501 bp | Woodford et al. (2006) |
bla OXA-24 | F: GGTTAGTTGGCCCCCTTAAA R: AGTTGAGCGAAAAGGGGATT |
52 °C | 246 bp | Woodford et al. (2006) |
bla OXA-48 | F: GCGTGGTTAAGGATGAACAC R: CATCAAGTTCAACCCAACCG |
58 °C | 438 bp | Poirel et al. (2011) |
bla OXA-51 | F: ATGAACATTMAARCRCTCTTACTTA R: CTATAAAATACCTAATTMTTCTAA |
50° C | 825 bp | Alcántar-Curiel et al. (2019b) |
bla OXA-58 | F: AAGTATTGGGGCTTGTGCTG R: CCCCTCTGCGCTCTACATAC |
52 °C | 599 bp | Woodford et al. (2006) |
mcr-1 | F: CGGTCAGTCCGTTTGTTC R: CTTGGTCGGTCTGTAGGG |
45° C | 309 bp | Liu et al. (2016) |