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. 2023 Mar 29;11:e15007. doi: 10.7717/peerj.15007

Table 1. Primers used for PCR amplification of β-lactamase and mcr-1 genes.

Gene Primer sequence (5′–>3′) T annealing (° C) Amplicon length Reference
bla TEM F: ATGAGTATTCAACATTTTCG
R: TTACCAATGCTTAATCAGTGAG
55 °C 861 bp Alcántar-Curiel et al. (2019b)
bla SHV F: ATGCGTTATATTCGCCTGTGTATT
R: TTAGCGTTGCCAGTGCTCGATC
60 °C 861 bp Alcántar-Curiel et al. (2019b)
bla CTX-M F: CGCTTTGCGATGTGCAG
R: ACCGCGATATCGTTGGT
52 °C 550 bp Alcántar-Curiel et al. (2019b)
bla KPC F: TCACTGTATCGCCGTCTAGTTCTG
R: TTACTGCCCGTTGACGCCCAATC
58 °C 875 bp Alcántar-Curiel et al. (2019b)
bla V IM F: GAGTGGTGAGTATCCGACAGTCAACGAAAT
R: AGAGTCCTTCTAGAGAATGCGTGGGAATCT
58 °C 389 bp Alcántar-Curiel et al. (2019b)
bla IMP F: GCATTGCTACCGCAGCAGAGTCTTTG
R: GCTCTAATGTAAGTTTCAAGAGTGATGC
58 °C 647 bp Alcántar-Curiel et al. (2019a)
bla NDM F: GTCTGGCAGCACACTTCCTATCTC
R: GTAGTGCTCAGTGTCGGCATCACC
58 °C 516 pb Alcántar-Curiel et al. (2019b)
bla OXA-23 F: GATCGGATTGGAGAACCAGA
R: ATTTCTGACCGCATTTCCAT
52 °C 501 bp Woodford et al. (2006)
bla OXA-24 F: GGTTAGTTGGCCCCCTTAAA
R: AGTTGAGCGAAAAGGGGATT
52 °C 246 bp Woodford et al. (2006)
bla OXA-48 F: GCGTGGTTAAGGATGAACAC
R: CATCAAGTTCAACCCAACCG
58 °C 438 bp Poirel et al. (2011)
bla OXA-51 F: ATGAACATTMAARCRCTCTTACTTA
R: CTATAAAATACCTAATTMTTCTAA
50° C 825 bp Alcántar-Curiel et al. (2019b)
bla OXA-58 F: AAGTATTGGGGCTTGTGCTG
R: CCCCTCTGCGCTCTACATAC
52 °C 599 bp Woodford et al. (2006)
mcr-1 F: CGGTCAGTCCGTTTGTTC
R: CTTGGTCGGTCTGTAGGG
45° C 309 bp Liu et al. (2016)