FIGURE 1.

Reproducibility of human IL-12 production interindividual variations. LPS-induced IL-12p70 production in whole blood cultures ex vivo from seven healthy subjects, as determined by ELISA and described in Materials and Methods. Experiments with whole blood cultures from different individuals were repeated two to seven times within several weeks, months, and, in some cases, within 2–3 years. To avoid circadian variations of cytokine production, experiments were performed between 2 and 3 p.m. Raw data for the cytokine production in pg/ml are depicted for each subject.