Figure 1.

Graphical timelines of experimental design for early-intervention and rescue paradigms. Diabetes was induced by intraperitoneal injection of streptozotocin (180 mg/kg) and confirmed three days later by blood glucose measurement. Sensory behavioral testing was performed at baseline and biweekly following diabetes induction. (A) Mice in the early-intervention paradigm were administered a ketogenic diet three weeks post-injection or maintained on a chow diet. Blood biomarkers were assessed biweekly and at sacrifice. (B) Mice in the rescue paradigm had uncontrolled diabetes for nine weeks before randomization into three groups: immediate sacrifice for peripheral innervation assessment, maintenance on chow diet, or administration of a ketogenic diet for four weeks. Blood biomarkers were assessed every four weeks.