Figure 6.

Intraepidermal innervation of diabetic footpads following using a ketogenic diet as a preventative intervention. (A) Representative images of fixed footpads fluorescently labeled with anti-protein gene product 9.5 (PGP9.5) to stain all nerve fibers. Scale bar set to 50 μm. (B) Quantification of PGP9.5⁺ fibers/mm. Diabetic mice exhibit approximately 30% fewer IENFs compared to non-diabetic controls, whereas diabetic mice fed a ketogenic diet exhibit normal IENFD (n=4). 2-Way ANOVA and Tukey’s post hoc test; * p < 0.05 compared to chow-fed non-diabetic mice.