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. 2023 Apr 1;14:49. doi: 10.1186/s40104-023-00843-2

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Schematic description of sample preparation and metabolomics analysis. a Bison were finished on rangeland pasture (pasture-finished) or fed free choice meadow hay, alfalfa hay, and whole shelled corn (pen-finished). b Meat samples (striploins) were cooked and ground, and extractions were performed using the automated MicroLab STAR® system. c Subsequent metabolomic analysis was conducted via LC-MS/MS. d Metabolites were identified using data extraction and peak identification software, and (e) data visualization tools including Metabolon Pathway Explorer, ChemRICH, and Metaboanalyst were used to inform (f) data interpretation including potential bioactivities and health effects of identified metabolites