FIGURE 1.

The effect of CD30 stimulation on intracellular reactive oxygen species (ROS) in adult T‐cell leukemia/lymphoma (ATL) cell lines. (A) Histogram of fluorescence intensity of an intracellular ROS indicator, CellRox. HUT102 cells were treated with the ROS inducer tert‐Butyl hydroperoxide (tTBHP; 50 μM) (left middle) and the ROS scavenger N‐acetyl‐L‐cysteine (NAC) (1 mM) (left bottom). The median fluorescence intensity (MFI) of each treatment condition is shown as a bar graph (right). Data are shown as mean ± SD of five independent experiments. Asterisks indicate statistical significance (*p < 0.05, ***p < 0.001). (B) HUT102 cells were treated with the ROS inducer TPA (50 nM) and the ROS scavenger glutathione (GSH) (1 mM). The MFI of each treatment condition is shown as a bar graph. Data are shown as mean ± SD of three independent experiments. Asterisks indicate statistical significance (**p < 0.01, ***p < 0.001). (C) Histogram of fluorescence intensity of CD30 expression (red) in ATL cell lines (HUT102 and TL‐Om1), ALL T‐cell lines (Jurkat and CEM), and a Burkitt lymphoma cell line (Ramos). Control staining by an isotype control antibody is shown in gray. (D) The MFI of CellRox in stimulated cells is shown as a bar graph. Data are shown as mean ± SD of three independent experiments. Asterisks indicate statistical significance (**p < 0.01). (E) Effect of CD30 stimulation on annexin V binding on the plasma membrane. The proportion of annexin V⁺ HUT102 and TL‐Om1 cells is shown as a bar graph. Data are shown as mean ± SD of three independent experiments. n.s., not significant