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. 2023 Jan 31;114(4):1616–1624. doi: 10.1111/cas.15707

FIGURE 1.

FIGURE 1

Study workflow. Neuroblastoma circulating tumor cells (CTC) were isolated from eight out of 10 patients with neuroblastoma at diagnosis and three out of four patients at relapse. In one patient (case 6), CTCs were isolated at both diagnosis and relapse. In another patient (case 4), CTCs were isolated at diagnosis before any treatment but not after intensive chemotherapy. Furthermore, whole genome amplifications were performed in 45 single CTCs from the five patients with neuroblastoma (cases 6–10). Among them, 14 single CTCs (31.1%) met the quality check (QC) standards. DNA sequencing targeting 50 cancer‐related genes and MYCN status evaluation using digital PCR at single‐cell level were performed. In total, whole transcriptome amplifications were performed on 30 single neuroblastoma cells from biopsied tumor tissues (primary tumor cells: PTC) and 48 single CTCs. Then, RNA sequencing of PTCs and CTCs at single‐cell level was performed.