Fig. 1.

Classification of intestinal organoids. (A) Schematic depicting the formation of intestinal organoids from crypts isolated from the intestine and embedded in Matrigel. Embedded crypts initially form rounded cyst-like structures. Within the first days of culture, these cystic structures polarise (Serra et al., 2019) and develop into the crypt and villus domains, also known as buds (Early organoid). Typically during days 3-6, more crypt-budding events occur (Late organoid). Under certain conditions, isolated crypts do not develop into budding organoids but acquire a spheroid morphology (Spheroid). Green cells represent stem cells. Illustration created with BioRender.com. (B) Summary of organoid classes, their description and corresponding labels in Tellu output. (C) Representative image showing the four organoid classes; boxed areas are shown magnified (x3) on the right. The arrowhead indicates a developing crypt. Scale bar: 500 μm.