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. 2023 Jan 27;14(2):915–929. doi: 10.1002/jcsm.13177

Figure 6.

Figure 6

hucMSCs alleviate PA‐induced C2C12 myotube atrophy via promoting AMPK‐mediated autophagy. (A) Western blot analysis of Atrogin 1 and MuRF1, p‐AMPK (T172) and p‐ULK1 (S555), LC3 and p62 in C2C12 myotubes treated with PA and hucMSCs (n = 3–4). (B) Diameters of C2C12 myotubes treated with PA and hucMSCs. (C) Western blot analysis of p‐ULK1(S555), LC3 and p62, Atrogin 1 and MuRF1 in C2C12 myotubes treated with hucMSCs and autophagy inhibitor 3‐MA (n = 4). (D) Diameters of C2C12 myotubes treated with hucMSCs and 3‐MA. (E) Western blot analysis of p‐AMPK (T172) and p‐ULK1 (S555), LC3 and p62, Atrogin1 and MuRF1 in C2C12 myotubes transfected with AMPK siRNA and treated with hucMSCs (n = 3–4). (F) Diameters of C2C12 myotubes transfected with AMPK siRNA and treated with hucMSCs. Quantification of bands was performed using ImageJ software. Data are mean ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001 using one‐way ANOVA followed by Tukey's test).