Skip to main content
. 2023 Feb 28;14(2):1060–1074. doi: 10.1002/jcsm.13194

Figure 2.

Figure 2

Dietary restriction of phosphate prevents the fibre cross‐sectional area (FCSA) reduction observed in old mice. Five‐month‐old mice (Young), 24‐month‐old mice fed with a standard diet (Old‐24m) and 24‐month‐old mice fed with a low‐phosphate diet (Old‐LowP) were evaluated. (A) Haematoxylin and eosin staining was performed in gastrocnemius muscle to measure the FCSAs (B). (C) Succinate dehydrogenase staining was also performed in gastrocnemius muscle to calculate the percentage of oxidative fibres (D). The graphs represent the box plot from values obtained from 15 young mice, 13 old mice and 13 old mice fed with the hypophosphataemic diet. (E) Myomesin‐2 expression was analysed by western blot in quadriceps samples. A represent blot is shown in the left panel. The graph represents the box plot from values obtained from 15 young mice, 16 old mice and 16 old mice fed with the hypophosphataemic diet. *P < 0.05 versus Young, # P < 0.05 versus Old‐24m.