Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 1;13:5366. doi: 10.1038/s41598-023-32524-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

X10ht enables confocal and STED imaging of tubulin immunostained in U2OS cells. (A) Scheme describing the differences between the X10 and X10ht protocols. (B) Representative images of tubulin under confocal or (C) STED imaging, indicating the difference in fluorescence intensity in both methods, what is quantified in (D). Using X10ht, we observed that the fluorescence signal appeared as a continuous decoration of the microtubules, while with X10 the loss of fluorophores results in “broken” microtubules (white arrows). N = ~ 90 regions of interest (ROIs) from two gels for each condition. (E) Exemplary epifluorescence images of the same region imaged pre- and post-expansion (X10ht) using the same 10 × objective. The region of the detail image is indicated by a white rectangle, showing expanded cells with a 100 × objective. (F) Distortion analysis of aligned pre- and post-expansion images. N = 3 automated measurements. Except panel E, scale bars, here and elsewhere, are given including the correction by the expansion factor. Data are presented as single ROI data points, mean ± SD.