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. 2023 Mar 20;14:1148412. doi: 10.3389/fendo.2023.1148412

Figure 5.

Figure 5

CRISPR gene knockout tool for screening immunotherapy-related genes. (A) By knocking out the ILK gene, cancer cells become more sensitive to immune cells. The expression of ILK is correlated with the expression level of PD-L1, which can be used as a therapeutic target for PD-L1-related immune escape. (B) In the prophylactic tumor inoculation model, the number of immunogenic necrotic cells was higher than that of apoptotic cells after AH1 knockdown. Necrosis may be the preferred form of ICD in cancer therapy. (C) SIAE is a gene that regulates the acetylation of Neu5AC. SIAE gene knockout can improve the acetylation level of Neu5AC and enhance the killing ability of NK cells. (D) TRAIL can kill CD133-positive tumor stem cells and reduce the number of M2 macrophages. The expression level of CD133 decreased after treatment. TRAIL can be used as a therapeutic drug for CD133 (+) tumor stem cells.