Table 2.
CRISPR/Cas9 techniques in new hot spots colon Cancer.
| Type | Gene modified | Disease model | References |
|---|---|---|---|
| metabolism genes | colon cancer | ||
| knockout | LDHA and LDHB; NAT1; SDHB; VDR; PPIP5KS; NOXO1; PLAUR; PPIP5K; NHLRC2 | (26–32, 139, 140) | |
| immune therapy | colon cancer | ||
| knockout | ILK; AH1; SIAE; CD133 | (66, 68, 141, 142) | |
| gene editing | CXCR2 and IL-2 | (84) | |
| Inflammatory bowel disease | colon cancer | ||
| knockout | Wnt-1; NHE8; P-cadherin; IL-6; SPHK1; IDO-1 | (60–64, 67) | |
| gene editing | P53 | (81) | |
| CRISP library | LGALS2; NFKBIZ, ZC3H12A and PIGR;/; TRAF5 | (110–113) | |
| Epithelial-mesenchymal transformation | colon cancer | ||
| knockout | Nrp2; Tks4; ALDOB | (69, 70, 143) | |
| knockin | p53 R273H | (71) | |
| SNPs linkage imbalance | colon cancer | ||
| knockout | SNPs Colon cancer risk genes; | (72) | |
| gene editing | rs4779584 and rs1406389; rs34149860; rs6854845 | (99–101) |