Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 30;220(6):e20221472. doi: 10.1084/jem.20221472

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Gonzalez et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — TAMs have distinct phenotypes after phagocytosis in acute lung tumor mouse models. (A) Schematic of intravenous transplantation of a primary lung tumor cell line (KPT; SP110P) into recipient F1 C57BL/6J-129S1/Svlmj mice over a 3-wk time course. (B) Flow cytometry gating of CD45^pos tdTom^pos and tdTom^neg lung macrophages gated on cells at 1, 2, and 3 wk after i.v injection of KPT primary lung tumor cell line. (C) Ordinary one-way ANOVA (*, P ≤ 0.05; ***, P ≤ 0.001). Bar plots showing the percentage of CD45^pos tdTom^pos cells in the acute lung tumor model after i.v. injection. (D) Myeloid cell composition within the tdTom^pos or tdTom^neg immune cell fraction in naive and tumor-bearing mice. (E and F) Fold change MFI of immune-modulatory and phagocytosis cell surface markers on IMs (E) or AMs (F) relative to naive IMs or AMs, respectively. Unpaired t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001). (G–J) Pathway enrichment score of immune-activation gene modules (G and H) and tissue-reorganization gene modules (I and J) for tdTom^pos or tdTom^neg IMs and AMs based on analysis of focused panels of Nanostring mRNA probes. Unpaired t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001). Data are representative of at least three independent experiments.