Figure S1.

Identification of phagocytic versus transduced TAMs in syngeneic and autochthonous lung tumors in vivo. (A) Heatmap of immune cell frequencies within naive or syngeneic tumor-bearing mice. (B) MFIs of cell surface markers on tdTom^pos or tdTom^neg BMDMs co-cultured with KPT SP110 cancer cells. (C) Schematic of lentiviral lung tumor initiation in Kras^stopflG12D Rosa26^{stopfl-tdTomato} mice. Ordinary one-way ANOVA (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001). (D) Flow cytometry plot of total live cells isolated from KT tumor-bearing mice. (E) Pie charts of the frequencies of myeloid cell subsets gated on tdTom-negative (tdTom^neg), tdTom-intermediate (tdTom^int), or tdTom-bright (tdTom^bright) CD45^pos CD11b/c^pos cells. (F) Flow cytometry plots of tdTom^bright, tdTom^int, and tdTom^neg lung macrophages gated on CD64^pos CD24^neg cells at in autochthonous lung tumors. (G) Scatter plots of the frequencies of tdTom^neg, tdTom^int, or tdTom^bright AMs and lung weights with linear regression calculations. (H) Fluorescent cell imaging of AMs from autochthonous lung tumors sorted from tdTom^int or tdTom^bright. Scale bar, 10 μm. (I) Flow cytometry plots of total macrophages gated on CD64^pos CD11b/c^pos CD45^pos cells isolated from lungs of KT^ai65;Spc^CreER mice transduced with indicated viral vectors. (J) Frequencies of tdTom^pos macrophages from mice in I. Data are representative of at least three independent experiments.