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. 2023 Mar 30;220(6):e20221472. doi: 10.1084/jem.20221472

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© 2023 Gonzalez et al.

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Figure S2. — Phagocytic TAMs have distinct cell surface phenotype in adenoviral autochthonous lung tumors in vivo. (A) Schematic of adenoviral lung tumor initiation in Kras^LSL-G12D Trp53^fl/fl Rosa26^LSL-tdTomato mice. (B) Flow cytometry plots of tdTom^pos lung macrophages gated on CD64^pos CD24^neg cells isolated from autochthonous lung tumors. (C) Stacked bar graph of the frequencies of myeloid cell subsets gated on tdTom^pos or tdTom^neg CD45^pos CD11b/c^pos cells in Adeno-SPCcre tumors. (D and E) MFIs of cell surface markers on tdTom^pos or tdTom^neg IMs (D) or AMs (E) from autochthonous Ad-SPCcre lung tumors. Ordinary one-way ANOVA (*, P ≤ 0.05; **, P ≤ 0.01). Data are representative of at least three independent experiments.