Skip to main content
. 2023 Mar 10;32:111–126. doi: 10.1016/j.omtn.2023.03.003

Figure 3.

Figure 3

Gint4.T-STAT3 inhibits NSCLC cell viability and migration induced by CAF CM

(A and B) Calu-1 cells were grown in 1% FBS medium (−) or in 1% CM from CAF#16 or #17 for 72 h in the absence (NT) or in the presence of 400 nmol/L Gint4.T, Gint4.T-STAT3, or CtrlApt-STAT3. (A) Cell lysates were analyzed by immunoblot with pSTAT3 and STAT3 antibodies. βActin or Vinculin antibodies were used as loading control. (B) Cell viability was measured and expressed relative to cells grown in 1% FBS medium (−). Error bars depict SD values (n = 2). (C–F) Calu-1 cells were grown in 1% FBS medium (−) or in 1% FBS CAF CMs for 24 h and then allowed to migrate using using Boyden chambers (8 μm pore size) uncoated (C and D) or coated with matrigel (E and F) using the correspondent medium as chemoattrattant. (C and E) Representative photographs are shown. (D and F) The percentage of migrated cells expressed relative to cells grown in 1% FBS medium (−) is reported. Error bars are SD values (n = 2). In (B), (D), and (F), statistics by t test are reported: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.