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. 2023 Mar 10;32:111–126. doi: 10.1016/j.omtn.2023.03.003

Figure 5.

Figure 5

CAF CM effects on NSCLC primary cultures

(A and B) Indicated primary NSCLC cells were grown in 5% FBS medium (−) or in 5% FBS CM from CAF#16 or #17 for 72 h in the absence (NT) or in the presence of 400 nmol/L Gint4.T, Gint4.T-STAT3, or CtrlApt-STAT3. Cell viability was measured and expressed relative to cells grown in 5% FBS medium (−). Error bars depict SD values (n = 2). (C and D) Migration of primary NSCLC cells grown in 5% FBS medium (−) or in 5% FBS CAF CMs was analyzed by Boyden chambers (8-μm pore size) containing the correspondent medium as chemoattractant. Left panels, representative photographs of migrated cells; right panels, quantization of migrated cells expressed relative to cell grown in 5% FBS medium (−). Error bars: SD values (n = 2). In (A)–(D), statistics by t test are reported: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.