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. 2023 Mar 10;32:111–126. doi: 10.1016/j.omtn.2023.03.003

Figure 7.

Figure 7

Gint4.T-STAT3 direct effect on CAFs

(A) Indicated CAFs were left untreated (NT) or treated with 400 nmol/L Gint4.T, Gint4.T-STAT3, or CtrlApt for 72 h and cell viability was measured and expressed relative to NT. (B) CAFs were left untreated (NT) or treated with 400 nmol/L Gint4.T, Gint4.T-STAT3, or CtrlApt-STAT3 and following 24 h were seeded in the Boyden upper chamber (8-μm pore size) and exposed to 10% FBS medium (lower chamber). Left panels, representative photographs of migrated cells are shown; right panels, migrated cells were quantified and expressed relative to NT. In (A) and (B), error bars depict SD values (n = 2). Statistics by t test (vs. NT) are reported: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. (C and D) Collagen contraction ability of CAFs left untreated (NT) or treated as indicated. Left panels, Representative pictures of plugs at different time points; right panels, plug areas measured by ImageJ and expressed as fold over untreated samples. Means and SD of two independent experiments are reported. Statistics by two-way ANOVA (vs. NT): ∗p < 0.05; ∗∗p < 0.01. (E and F) Indicated CAF markers were analyzed by RT-qPCR in primary CAFs treated with 400 nmol/L CtrlApt or Gint4.T-STAT3. Error bars depict SD values (n = 2). Statistics by t test are reported: ∗p < 0.05; ∗∗p < 0.01.