Fig. 1.

Effects of high‐fat diet on body weight, daily food intake, liver weight, and different abdominal adipose tissues in mice. (A) Comparison of the body weight between the control diet (CD) and high‐fat diet (HFD) mice from 4 to 14 weeks of age (n = 20). (B–D) Comparison of BMI, daily food intake, and energy intake in CD and HFD mice at 14 weeks of age. (E) Serum lipids detection. (F–H) Fasting blood glucose, 2‐h postprandial blood glucose levels, serum insulin levels, and HOMA‐IR in mice. (I) The ratio of the weight of mesenteric, inguinal, retroperitoneal, and epididymal adipose tissue to body weight, respectively, in CD and HFD mice at 14 weeks of age (n = 6). (J) Liver weight. (K) Oil Red O staining of liver tissues. Scale bars, 200 μm. (L) The levels of total protein, albumin, ALT, and AST in mouse serum (n = 6). Data are expressed as mean ± SD. Statistical analyses were performed using the two‐way ANOVA (A) and Student's t‐test (B–J, L) NS, no significant difference. **P < 0.01, ***P < 0.001. ALT, alanine aminotransferase; AST, aspartate transferase; BMI, body mass index; CHOL, cholesterol; HDL‐C, high‐density lipoprotein cholesterol; HOMA‐IR, homeostatic model assessment for insulin resistance; LDL‐C, low‐density lipoprotein cholesterol; TG, triglycerides.