Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 14;13(4):763–778. doi: 10.1002/2211-5463.13589

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 4 — Mitochondrial structural and functional analyses of mouse sperm. Mitochondrial membrane potential (MMP) of sperm in mice based on measurement of the JC‐1 red/green fluorescence ratio (A) and TMRM staining (B and C, CCCP as a positive control). (D) The ratio of si‐DMA fluorescence intensity to TMRM fluorescence intensity. (E) Mitochondrial morphology of sperm from cauda epididymis in CD and HFD mice was observed by transmission electron microscope (TEM). Scale bars, 200 nm. (F) Statistical analysis of sperm mitochondrial diameters. (G) Comparison of sperm ATP content between CD and HFD mice. (H) Comparative proteomics analysis of differentially expressed proteins (DEPs) relevant to mitochondrial function in sperm between CD and HFD mice. Data are expressed as mean ± SD (n = 10). Statistical analysis was performed using the Student's t‐test. *P < 0.05; **P < 0.01. JC‐1, tetrechloro‐tetraethylbenzimidazol carbocyanine iodide; TMRM, tetramethyl rhodamine methyl ester.