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. 2023 Feb 10;42(7):e112699. doi: 10.15252/embj.2022112699

Figure 6. Overview of key nuclear maturation events of the large ribosomal subunit in yeast.

Figure 6

Cryo‐EM structures of yeast pre‐60S particles at different stages of maturation (PDB ID: 6ELZ, 3JCT, 6YLG, 6N8J). RBFs involved in these steps are color‐coded, as well as the pre‐rRNA (white), L1 stalk (red), and 5S rRNA (orange). The nucleolar pre‐60S in state E shows a displacement of the L1 stalk from its position in the mature subunit. The successive nucleoplasmic Arx/Nog2 particle is a result of a stepwise release and binding of the indicated RBFs and the 5S RNP. The Rix‐Rea1 remodeling machinery initiates the formation of the central protuberance (CP) and rotation of the 5S RNP to its mature conformation, visible in the successive late nuclear (LN) particle.