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. 2023 Feb 10;42(7):e112699. doi: 10.15252/embj.2022112699

Figure 7. Cytoplasmic maturation of 60S subunit in yeast.

Figure 7

Cryo‐EM structures of pre‐60S particles illustrating snapshots of cytoplasmic maturation events (PDB ID: 6N8L, 7Z34, 6N8M, 6N8N, 6RZZ, 6QTZ). RBFs are color‐coded, as well as the pre‐rRNA (white), L1 stalk (red), and 5S rRNA (orange). After nuclear export, the ATPase Drg1 dissociates Rpl24 from early cytoplasmic pre‐60S particles. The release of multiple factors allows the association of the GTPase Lsg1, which in turn dissociates Nmd3 upon the incorporation of Rpl10/uL16. Following Reh1 dissociation, the GTPase Efl1 together with Sdo1 triggers the release of Tif6, resulting in mature 60S subunits.