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. 2023 Feb 8;29(5):1254–1271. doi: 10.1111/cns.14099

FIGURE 1.

FIGURE 1

miR‐26a‐5p alleviates complete Freund's adjuvant‐induced inflammatory pain and suppresses inflammation by a single intrathecal or subcutaneous injection. (A) Experimental protocol for establishing complete Freund's adjuvant‐induced inflammatory pain mouse model, tissue collection, and pain behavioral test. (B) 50% paw withdraw threshold (PWT) of left hind paw of mice in different treatment groups mice. At day 1, the paw withdrawal thresholds (WTs) of CFA/miR(s.c.) group were significantly higher than those of Sham group after single injection. At day 1, 3, 5 and 7, the WTs of CFA/miR(i.t.) group were significantly higher than those of Sham group after single injection. (C) Thermal latency(s) of left hind paw of mice in different treatment groups. At day 1, the thermal latency(s) of CFA/miR(s.c.) group was significantly higher than those of Sham group after single injection. At day 1, 3, 5 and 7, the thermal latency(s) of CFA/miR(i.t.) group was significantly higher than those of Sham group after single injection (*p < 0.05, **p < 0.01 compared with CFA/miR(i.t.) and CFA/miR(s.c.) group by two‐way ANOVA followed by Tukey's post hoc test, n = 8 in each group). (D, E) mRNA levels of IL‐1β, IL‐6, TNF‐α, IL‐10, and TGF‐β after injection of miR‐26a‐5p agomir. Statistical significance of mean differences was determined with the Tukey's multiple comparisons test.