PCR1 reaction master mix
| Reagent | Volume (μL) |
|---|---|
| gDNA template (10 μg) | x |
| Herculase II polymerase | 2 |
| oMCB_1562 (100 μM) | 1 |
| oMCB_1563 (100 μM) | 1 |
| 5× Herculase buffer | 20 |
| dNTPs (100 nM)∗ | 1 |
| ddH2O | 75-x |
∗25 nM per dNTP.
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