PCR2 reaction master mix
| Reagent | Amount (μL) |
|---|---|
| PCR1 product | 5 |
| Herculase II polymerase | 2 |
| oMCB_1440 (100 μM) | 0.8 |
| oMCB_1439 (100 μM) | 0.8 |
| 5× Herculase buffer | 20 |
| dNTPs (100 nM) | 2 |
| ddH2O | 69.4 |
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