Fig. 1.

Transferrin and folic acid receptors are highly expressed in pancreatic ductal adenocarcinoma. A) Representative Western blot analysis of transferrin receptor, folic acid receptor, and epidermal growth factor receptor from whole protein lysates of the normal immortalized pancreatic ductal cell line HPNE and a panel of PDAC cells (MIA PaCa-2, PANC-1, HS766t, 4671-T). Cell line 4671-T-CRC (4671-T) is a conditionally reprogrammed cell line from a primary PDAC tumor generated and gifted by Dr. Rosalie Sears from Oregon Health & Sciences University. Protein quantification depicted under each band was normalized to β-actin and relative to HPNE. B) Quantification of A. Average of relative protein expression ± standard error of the mean (n = 4). Statistical analysis was calculated using multiple Student's two-sample t-test. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05, n.s. not significant. C) Representative immunohistochemistry staining for transferrin receptor, folic acid receptor, and epidermal growth factor receptor of patient tissues, both normal and PDAC, as well as the full panel of tissues.