Fig. 2.

FA-3DNA and Tf-3DNA increase targeting of 3DNA to PDAC tumors, in vivo. A) Images of tumor-bearing mice over time after treatment with folic acid-conjugated 3DNA (FA-3DNA), transferrin-conjugated 3DNA (Tf-3DNA), oligo control 3DNA (Oligo-3DNA), or no treatment (NT). Images taken at 36 h are not shown. Bioluminescence of tumors are shown in the left panels. 3DNA was monitored over time by fluorescence, as seen in the right panels. Bioluminescence and fluorescence intensity for each image is to the same scale. B) Quantification of the fluorescence depicted in A. C) qPCR detection of 3DNA in vascular circulation at indicated time points. Serum was extracted from blood collected at time points and used for 3DNA qPCR quantification. Values were normalized to 18S. Statistical analysis of time course data presented in B and C were calculated using a Student's two-sample t-test of the area under the curve. **p<0.01. D) qPCR detection of 3DNA in homogenized organs 3 h after re-injection of the same treatment. Right graph depicts only pancreas samples. Values were normalized to 18S. Statistical analysis was calculated using two-way ANOVA. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05, n.s. not significant. n = 3 mice for each experimental arm.