Fig. 3.

3DNA clears quickly without the presence of a tumor. A) Representative images of both tumor- and non-tumor-bearing mice over time after treatment with folic acid-conjugated 3DNA (FA-3DNA) or unconjugated 3DNA (UN-3DNA). Mice not treated (NT) are not pictured. Tumors are shown by bioluminescence in the left panels. 3DNA was monitored over time by fluorescence, as seen in the right panels. B) Quantification of the fluorescence depicted in A. Statistical analysis was calculated using a Student's two-sample t-test for each treatment at each time point in comparison to NT mice. Significance is indicated at the bottom of the graph. C) qPCR detection of 3DNA in homogenized organs 7 h after re-injection of the same treatment. Values were normalized to 18S. Statistical analysis was calculated using a two-way ANOVA. Brackets mark significance between organs and treatments. Bars mark significance between treatments of the same organ. Asterisks directly above of bars indicate significance between tumor-bearing organs and non-tumor-bearing organs. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05, n.s. not significant. n = 3 mice for each experimental arm. Bioluminescence and fluorescence intensity for each image is to the same scale.