Fig. 1.

Time course for the stabilization of p53 by bortezomib in mouse bone marrow. (A) The schedule of bortezomib (BTZ) time course experiments (n = 3 to 4). p0, neonatal; wk, week, h, hour; p.i., intraperitoneal injection. (B) Quantification of p53-positive frequencies at various time points from mice treated with vehicle (BTZ 0 h) or BTZ. Moderate and strong p53 staining signals are classified as p53 2-3+ (in brown), mild p53 staining signals are classified as p53 1+ (in light brown). The indicated P values were calculated by multiple unpaired t tests using Prism. *P < 0.05; **P < 0.001. Error bars represent the mean (SEM) for each group. (C) IHC analysis of p53 in bone marrow from mice at indicated time points after 3 mg/kg BTZ treatment. Purple arrow points to detectable p53 in cells treated with vehicle, indicating high p53 activity in the bone marrow. (Scale bars, 20 μm.)