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. 2023 Mar 20;120(13):e2219978120. doi: 10.1073/pnas.2219978120

Fig. 3.

Fig. 3.

p53 target gene expression and apoptosis induction in bone marrow following bortezomib treatment. (A) The schedule of bortezomib (BTZ) experiments for detecting p53 target gene and apoptotic induction (n = 3 to 4). p0, neonatal; wk, week, h, hour; p.i., intraperitoneal injection. (B) qRT-PCR analysis of Cdkn1a, Puma, Gadd45a, Trp53, and Ccna2 expression in the bone marrow of p53wt and p53ko mice treated with BTZ (3 mg/kg, 3 h). (C and D) IHC analysis of p21 and Puma in the bone marrow from mice at indicated time points after vehicle (BTZ 0 h) or 3 mg/kg BTZ treatment. (Scale bars, 20 μm.) (E and E′) IHC analysis and quantification of the expression of cleaved caspase 3 in the bone marrow of p53wt and p53ko mice at indicated time points post vehicle (BTZ 0 h) or 3 mg/kg BTZ treatment (n = 3 to 4). (Scale bars, 20 μm.) (F) Quantification of the frequencies of HSPCs and various mature cells in the bone marrow from p53wt and p53mt mice (n = 9 to 10). For B, the indicated P values were calculated by two-way ANOVA using Prism. For C′ and D, the indicated P values were calculated using multiple unpaired t tests using Prism. *P < 0.05; **P < 0.001; ***P < 0.0001; ****P < 0.00001, ns, no significance. The error bars represent the mean (SEM) for each group.