Fig. 2.
NUP153 targets CA lattice interfaces in the assembled capsid. (A) SEC coelution assays of CA hexamer (Left), tri-hexamercenter (Middle), or pentamer (Right) with NUP1531411–1475. Top: SDS-PAGE of eluates. Only the tri-hexamercenter induced a notable peak shift of NUP1531411–1475, indicative of complex formation, with a corresponding shift of eluted NUP1531411–1475 protein by SDS-PAGE (Top). Independent experiments were repeated three times, with representative examples shown. (B) SEC-MALS analysis of NUP1531401–1475 and tri-hexamercenter. Overlay of the individual SEC-MALS elution profiles of NUP1531401–1475 (green), tri-hexamercenter (red), and a mixture of the two (blue). The measured (black) and predicted (red) molecular weights are shown.