Fig. 6.
NUP153 stabilizes CA assemblies. (A) Negative-stain electron micrographs of intact WT CA tubes under high salt conditions (1 M NaCl, Left), disintegrated WT CA tubes under low salt conditions (75 mM NaCl, Middle), and WT CA tubes (6 µM CA) stabilized by NUP1531411–1475 (50 µM) under low salt conditions (75 mM NaCl, Right). (Scale bars: 100 nm.) (B) WT CA tubes (12 µM CA) incubated with 100 µM bipartite peptide (25 aa), FG-motif peptide (14 aa) and RRR-motif peptide (11 aa) under 150 mM NaCl. (Scale bars: 100 nm.) (C) Same assays as in (B), but with the peptides at 15 µM with WT CA tubes. (Scale bars: 100 nm.) All experiments were performed independently three times, with representative examples shown.