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. 2023 Mar 23;120(13):e2220984120. doi: 10.1073/pnas.2220984120

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Copyright © 2023 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 1. — The measurement of α-synuclein, tau, and Aβ prions in Guam ALS-PDC and sporadic AD brain extracts using a cellular bioassay panel. (A) Prion infectivity measurements in Guam ALS-PDC brain samples (PK+PTA extracts) using transfected HEK293T cells stably expressing the α-syn(A53T)-YFP (black), 3R tau(K19VM)-YFP (light blue), 4R tau(K18LM)-YFP (blue), YFP-Aβ40 (pink), or YFP-Aβ42 (red) fusion proteins (Materials and Methods). Comparison of prion infectivity in Guam ALS-PDC (filled circles) or sporadic AD (open circles) patient brain extracts in (B) 3R tau(K19VM)-YFP cells (light blue), (C) 4R tau(K18LM)-YFP cells (blue), (D) YFP-Aβ40 cells (pink), or (E) YFP-Aβ42 cells (red). Data shown are individual donors plotted with mean ± SD as determined from four images per well in four wells per donor sample.