Fig. 3.
LRH1 promoted PC cell migration and invasion. (A) Pro-migration of LRH1 on Capan-1 and AsPC-1 cells. LRH1 promoted Capan-1 and AsPC-1 cell Migration using BD Bioscience Chamber. Compared to vectors, LRH1 expressing stable Capan-1 and AsPC-1 cells showed enhanced cell migration at 24 h in Boyden chamber assay. A substantial increase in migration was present in LRH1 stable transfected Capan-1 and AsPC-1 cells compared to vector control (**p < 0.01). (B) LRH1 overexpression promoted Capan-1 and AsPC-1 cell invasion. Compared to vector control, LRH1 stable overexpressing Capan-1 and AsPC-1 cells conferred enhanced cell invasion at 24 h. Enhanced invasion was present in LRH1 stable transfected Capan-1 and AsPC-1 cells compared to vector control (**p < 0.01). Significant attenuation of cell migration (C) and invasion (D) was observed at 36 h in three stable MIA PaCa2 cell lines expressing LRH1-shRNAs (**p < 0.01).