Figure 1.
The structure of the p110γ-p84 complex and comparison with p110γ-p101
(A) Cartoon schematic of the PI3Kγ catalytic (p110γ) and regulatory subunits (p101 and p84) with domain boundaries indicated.
(B) Cartoon of differences in activation between p110γ-p84 and p110γ-p101 complexes downstream of GPCRs and RTKs.
(C) Structure of the p110γ-p84 complex based on X-ray crystallography, negative-stain EM, and AlphaFold modeling (PDB: 8AJ8, supporting data in Figures S1 and S2). Domains are indicated from (A), with a cartoon schematic shown in the bottom left.
(D) Structure of the p110γ-p101 complex (PDB: 7MEZ).32 Domains are indicated from (A), with a cartoon schematic shown in the bottom left.
(E and F) Differences in the C-terminal domain of p84 (E) and p101 (F) are shown with this domain shown as an electrostatic surface.