Fig. 2.

The effect of C3G on H/R-mediated ferroptosis in HK-2 cells. A Cell viability determined by CCK-8 cell viability assay. (n = 6). The cells were exposed to hypoxia and reoxygenation (H/R); B LDH release was assessed by LDH assay kit in different groups. (n = 6); C–E The levels of iron, MDA and GSH of different groups in HK-2 cells. (n = 6); F, G RT-PCR results of GPX4 and ACSL4 in HK-2 cells. (n = 6); H Western blot results of GPX4 and ACSL4 in HK-2 cells. (n = 3). *p < 0.05, **p < 0.001. C3G cyanidin-3-glucoside, H/R hypoxia/reoxygenation, HK-2 human proximal tubule epithelial cells, CCK-8 cell counting kit-8, LDH lactate dehydrogenase, MDA malondialdehyde, GSH glutathione, RT-PCR reverse transcription polymerase chain reaction, GPX4 glutathione peroxidase 4, ACSL4 acyl-CoA synthetase long chain family member 4