Fig. 3.

Treatment of C3G attenuated I/R-AKI in vivo. A Schematic of renal I/R generation and treatment of C3G for one week in advanced; B–D BUN, Scr levels and kidney coefficient. Kidney coefficient = Kidney weight/body weight × 100. (n = 6); E Representative micrographs of HE and PAS staining of kidney from sham + C3G, sham, I/R and I/R + C3G group. The pathological scores of kidney injury were graded. Scale bars = 20 μm. (n = 6); F, G Representative terminal deoxynucleotidyl transferase dUTP nick-end label- (TUNEL-) stained sections of different groups in kidney. Scale bars = 20 μm. (n = 6); H, I Representative immunofluorescence images of ROS were captured and analyzed by the ratio of ROS positive cells/total cells. Scale bars = 20 μm. (n = 6). *p < 0.05, **p < 0.001. C3G cyanidin-3-glucoside, I/R-AKI ischemia/reperfusion-induced acute kidney injury, BUN blood urea nitrogen, Scr serum creatinine, HE hematoxylin and eosin, PAS Periodic acid-Schiff, ROS reactive oxygen species