Fig. 6.

The knockdown of AMPK abrogated the protective effect of C3G against ferroptosis. A The efficiency of knockdown of AMPK via si-AMPK was analyzed by Western blot. (n = 3); B, C The content of MDA and GSH were determined in HK-2 cells with or without C3G after knockdown of si-AMPK under H/R condition. (n = 6); D Western blot results of AMPK, P-AMPK, GPX4 and ACSL4 in HK-2 cells with or without C3G after knockdown of si-AMPK under H/R condition (n = 3). *p < 0.05, **p < 0.001. C3G cyanidin-3-glucoside, HK-2 human proximal tubule epithelial cells, AMPK AMP-activated protein kinase, MDA malondialdehyde, GSH glutathione; GPX4 glutathione peroxidase 4, ACSL4 acyl-CoA synthetase long chain family member 4, H/R hypoxia/reoxygenation