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Cell staining. A Crystal violet staining assay used for morphological evaluation of human osteoblast-like MG-63 cells (MG-63). B Alizarin staining used for localization and quantification of calcium deposits of bone marrow-derived mesenchymal stem cells (BM-MSCs). C ALP used as an early osteogenic marker of bone calcification (BM-MSCs). Photographed on microscope Olympus CKX41 with a ×10 magnification lens. Scale = 200 μm
