Table 1.
Genes and primers for monitoring osteodifferentiation
| Gene | Forward sequence | Reverse sequence |
|---|---|---|
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)—housekeeping | GAGTCCACTGGCGTCTTCAC | GTTCACACCCATGACGAACA |
| Runt-related transcription factor 2 (Runx2) | GTAGATGGACCTCGGGAACC | GAGCTGGTCAGAACAAAC |
| Osterix (Osx, Sp7) | TTCTGCGGCAAGAGGTTCACTC | GTGTTTGCTCAGGTGGTCGCTT |
| Alkaline phosphatase (ALP) | GGAACTCCTGACCCTTGACC | TCCTGTTCAGCTCGTACTGC |
| Collagen type I (Col1) | GAGTGCTGTCCCGTCTGC | TTTCTTGTTCGGTGGGTG |
| Bone sialoprotein (BSP) | GGCAGTAGTGACTCATCCGAAG | GAAAGTGTGGTATTCTCAGCCTC |
| Osteopontin (OPN) | GTTTCTCAGACCTGACATCC | CATTCAACTCCTCGCTTTCC |
| Osteonectin (SPARC, BM-40) | TGCCTGATGAGACAGAGGTGGT | CTTCGGTTTCCTCTGCACCATC |
| Osteocalcin (OCN) | GGCAGCGAGGTAGTGAAGAG | CTCACACACCTCCCTCCTG |
| Fibroblast growth factor-23 (FGF-23) | GGAACAGCTACCACCTGCAGAT | CACCACAAAGCCAGCATCCTCT |
| Bone morphogenetic protein-2 (BMP2) | CAGACCACCGGTTGGAGA | CCACTCGTTTCTGGTAGTTCTTC |
| Receptor activator of nuclear factor κB ligand (RANKL) | GCCTTTCAAGGAGCTGTGCAAAA | GAGCAAAAGGCTGAGCTTCAAGC |
| Osteoprotegerin (OPG) | GGTCTCCTGCTAACTCAGAAAGG | CAGCAAACCTGAAGAATGCCTCC |
| Sclerostin (SOST) | GGAGCTGGAGAACAACAAGACC | TCACGTAGCGGGTGAAGTGCAG |
According to ref. [65]