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. Author manuscript; available in PMC: 2023 Apr 3.
Published in final edited form as: Dev Dyn. 2022 Jun 2;251(10):1754–1773. doi: 10.1002/dvdy.499

Table 1. Summary of the advantages and disadvantages of Switch transgenes.

Comparison of the relative switching efficiencies and logistical considerations for experimental design using the different Switch transgene lines described.

Line Advantages Disadvantages
ubi:Switch Second highest recombination efficiency Cannot be combined with other EGFP lines
No need for heat-shock Latency in reporter expression
ubi:delta-EGFP Can be combined with other EGFP lines No screening marker
Same recombination efficiency as ubi:Switch Latency in reporter expression
hsp70l:Switch Highest recombination efficiency Need to heat-shock
Easy screening marker cryaa:Venus
Rapid transgene expression after heat-shock
actb2:BFP-DsRed Can be combined with other EGFP lines Low recombination efficiency
actb2:Stop-DsRed Can be combined with other EGFP lines Low recombination efficiency
No screening marker
Multiple integrations in current generation