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. Author manuscript; available in PMC: 2023 Apr 3.
Published in final edited form as: Mol Microbiol. 2021 May 18;116(2):550–563. doi: 10.1111/mmi.14732

FIGURE 2.

FIGURE 2

The first 50 amino acids of MucA are necessary and sufficient for viability. (a) Schematic of MucA encoded by the ectopic mucA alleles in PAO1 ∆algD strains tested for mucA essentiality in (b). MucA aa 1–143 lacks the MucB binding domain and is the product of the common mucA22 allele. MucA 1–110 lacks the entire periplasmic domain. MucA aa 1–75 contains most of the AlgU binding domain, while shorter truncations contain only parts of the AlgU binding domain (MucA aa 1–62, 1–50, 1–40, and 1–24). Green, AlgU binding domain (AlgU BD); blue, the transmembrane domain (TM); and purple, the MucB binding domain (MucB BD). (b) Frequency of observed isolates resolving to the endogenous mucA allele (WT, black) or the deletion allele (∆mucA, white) in the allelic exchange assay. Super-imposed on the bars are the number of isolates that were tested in each category. Asterisk, p < .0001; Fisher’s exact test. Caret, slower growing isolates