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ABP labeling in complex protein mixtures. A) Bone marrow cells were isolated, lysed, and incubated with Cy5-PK-DPP or Cy5-V-DPP at 1 or 10 μM. Proteins were resolved by SDS-PAGE and protease labeling was detected by measuring in-gel fluorescence. B) Immunoprecipitation of Cy5-V-DPP-labeled lysates in A with an elastase-specific antibody, followed by SDS-PAGE and in-gel fluorescence detection.
