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. Author manuscript; available in PMC: 2023 Apr 3.
Published in final edited form as: Bioorg Med Chem Lett. 2016 Nov 25;27(2):254–260. doi: 10.1016/j.bmcl.2016.11.064

Fig. 4.

Fig. 4.

Ex vivo labeling of protease activation during acute pancreatitis. A) Lysates were prepared from normal and inflamed pancreas and incubated with Cy5-PK-DPP or Cy5-V-DPP (10 μM). Proteins were resolved by SDS-PAGE and protease labeling was detected by in-gel fluorescence. B) Recombinant neutrophil elastase was incubated with Cy5-V-DPP (1 μM), either alone (rNE) or in the presence of pancreas lysates (normal/inflamed + rNE). Elastase activity was detected by in-gel fluorescence (top/red). Total elastase levels were then measured by western blot (bottom/blue). Bands were quantified by densitometry. (Activity: rNE v Norm p = 4.1e-5; rNE v Infl p = 1.4e-5; Norm v Infl p = 0.09. Total: rNE v Norm p = 0.0005; rNE v Infl p = 0.008; Norm v Infl p = 0.97. Norm Act v Tot p = 0.0003; Infl Act v Tot p = 0.0006).