Fig. 6.

In vivo imaging of pancreatitis with ABPs. A) Healthy mice or those with acute pancreatitis were injected with Cy5-PK-DPP, Cy5-V-DPP, or vehicle control (No Probe). After 5 h, pancreata were removed and imaged for Cy5 fluorescence. Gain settings are indicated to the right of each image series. B) Quantification of absolute levels of Cy5 fluorescence measured in A with background subtracted (no-probe control). C) Biochemical analysis of protease labeling in the tissues from A. Pancreata were lysed and proteins were resolved by SDS-PAGE. Protease labeling was detected by measuring in-gel fluorescence. Note autofluorescent background bands in the no-probe control.