Table 1.

Water and food intake

	Age (months)
	5	18–21	21–24	24–27
Time on intervention (months)	-	0 to 3	3 to 6	6 to 9
Water intake (mL day−1)
Control	3.8 ± 0.6	3.4 ± 0.7*	3.5 ± 0.8*	3.7 ± 0.7
DMB	-	2.3 ± 0.4†	2.6 ± 0.6†,^	3.2 ± 0.8†,^
Food intake (kcal day−1)
Control	12.4 ± 2.2	13.3 ± 2.8	11.8 ± 2.1	11.3 ± 2.4*
DMB	-	11.9 ± 2.0†	11.2 ± 2.7	11.3 ± 3.7

Data are mean ± SD. Food/water intake are reported as average intake per day in young mice (5 months; n = 12) over the course of 8 weeks, and older mice (18–27 months) over the course of the 3–9 months of intervention (averaged across each 3 month interval; mice were removed from the analysis as they were euthanized and studied at each time point; n = 12–41 mice per treatment group per 3 month interval). Mice were group housed, so food/water intake was determined as the total intake divided by the number of mice within a given cage. Statistics are one-way (effect of age in control mice only) ANOVA with Dunnett’s post hoc test and two-way mixed (age × treatment group) ANOVA with Šídák’s post hoc test.

^*P < 0.05 control vs. 5 month reference group.

^†P < 0.05 DMB vs. control within time point.

^{^}P < 0.01 vs. DMB aged 18–21 months. Abbreviations: DMB, 3,3-dimethyl-1-butanol.